anti cd80 apc  (Bioss)

Journal: Cells

Article Title: Cancer Immunomodulatory Effect of Bidens pilosa L. in Mice: Suppression of Tumor-Associated Macrophages and Regulatory T Cells

doi: 10.3390/cells15020126

Figure Lengend Snippet: Expression levels of mRNAs and proteins associated with TAMs and Tregs in BPA -treated mouse tumors. ( A ) Quantification of mRNA by qRT-PCR for TAM/Treg-associated tumor-promoting factors (Arg-1, YM-1, CCL2, CCL22, Foxp3, CD25, TGF-β, CXCR3, CCR4, CCR8, CCR10) and tumor-inhibitory factors (TNF-α, IL-1β, iNOS, CXCL10, CCR7). Data were normalized to 18 S rRNA and are shown relative to the model group (baseline control). ( B ) Representative immunoblots of tumor tissue for TAM- and Treg-associated proteins. Pro-tumor: CD206, Foxp3, CD25, PD-1, PD-L1; Anti-tumor: CD80, CD4, GITR. ( C ) Quantification of protein expression from Western blots. Values were normalized to α-tubulin, β-actin, or GAPDH as indicated. Statistical significance is denoted as follows ( n = 6): compared to the model group, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: To block Fc receptors, cells were incubated with anti-CD16/32 (Elabscience, Cat. E-AB-F0997A, Wuhan, China) for 10 min. After washing, cells were stained with PerCP/Cyanine 5.5 anti-mouse F4/80 (Elabscience, Cat. E-AB-F0995J), FITC anti-mouse CD206 antibody (Elabscience, Cat. E-AB-F1135C), and APC anti-mouse CD80 antibody (Elabscience, Cat. E-AB-F0992E) at 4 °C for 30 min.

Techniques: Expressing, Quantitative RT-PCR, Control, Western Blot